Lamin A/C is a protein present in all cells. Among its many functions, it also contributes to the normal organization of the cell’s nucleus. It helps anchor the DNA and keeps it in the correct place within the 3D space of the nucleus. The absence of lamin A/C leads to changes in the organization of the nucleus, including changes in positions of the genetic material in the nucleus, slower cell growth, early-onset of ageing and early cell death. Lamin A/C was described as one factor that is upregulated in prostate cancer when the cancer becomes aggressive and forms metastatis. However, no knowledge exists about lamin A/C in circulating tumor cells (CTCs) and in relation to disease aggressiveness that may be predictable using CTCsand liquid biopsies (instead of tissue biopsies). We have previously characterized the stability or aggressive behaviour of prostate cancer CTCsusing 3D imaging of telomeres. Our data suggest a very good stratification of patients into stable or aggressive. We hypothesize that lamin A/C may make this classification even more accurate and propose to test it in parallel with 3D telomere profiling in CTCs of prostate cancer patients of low, intermediate, and high risk.