Cisplatin, one of the most potent and widely used anticancer drugs, is being evaluated for chemotherapy of advanced metastatic prostate cancer where hardly any effective treatment options exist. The focus of our research is on the mechanism of cisplatin detoxification by the Wilson disease protein (ATP7B) and interplay between various copper transporters in the pathways of cisplatin delivery to the target on the one hand, and drug detoxification on the other. The goal of this project is to investigate if copper carrier Atox1 potentiates pharmacological activity of cisplatin by delivering it directly to DNA in the cell nucleus, and to determine the role of copper transport proteins in modulating prostate cancer sensitivity to cisplatin. Our investigation of the interplay between copper andplatinum transport in the cell will lead to more effective and less toxic chemotherapy protocols for treating advanced prostate cancer with platinum drugs.